Polymerase chain reaction (PCR) technique has been commonly employed in the aquaculture industry for disease diagnosis and prevention. With the growing demand for a quantitative research tool and requirement for a high throughput screening system, the real-time PCR is receiving more attention and becoming more important in the industry. IQ REAL™ Quantitative System is a real-time PCR based diagnostic system which not only meets the above demands, but also its advanced design provides the shrimp culture industry a more accurate, more sensitive, and more flexible real-time system.